Regensburg 2010 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 7: Electronic and Optical Properties of Organic Systems II
CPP 7.2: Vortrag
Montag, 22. März 2010, 14:15–14:30, H39
Time resolved 3D orientation spectroscopy - experimental realisation and simulation — •Richard Börner1, Danny Kowerko2, Christian von Borczyskowski2, and Christian G. Hübner1 — 1Institute of Physics, University of Lübeck, 23538 Lübeck, Germany — 2Center for Nanostructured Materials and Analysis, Chemnitz University of Technology, 09107 Chemnitz, Germany
Confocal microscopy is a powerful tool for single molecule investigation of fluorescent macromolecules. Besides the common studied features in single molecule detection, the 3D orientation determination of the emission dipole enables the analysis of different conformational states. These conformational states can be represented as state depending dipole orientations intrinsic to the fluorescent molecule and/or in relation to the molecular frame. Furthermore, conformational states can be subject to intramolecular dynamics which may lead to spectral diffusion, fluorescence intensity and/or lifetime fluctuations and changes in the orientation of the emission dipole. Therefore, a simultaneous measurement of all parameters is mandatory. To this end, we show a detection scheme that allows for simultaneous determination of the full 3D emission dipole orientation, fluorescence intensity, the fluorescence lifetime and the emission spectra of single fluorescent molecules. We demonstrate the feasibility of our approach using pyridyl functionalized perylene bisimide (PBI). Moreover, monte-carlo simulations demonstrate the full potential of our detection scheme to quantify rotational dynamics on different time scales, in particular under confinement conditions.