Regensburg 2010 – wissenschaftliches Programm
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ST: Fachverband Strahlen- und Medizinphysik
ST 10: Basic and Applied Medical Physics (Poster Session)
ST 10.4: Poster
Mittwoch, 24. März 2010, 16:00–17:00, H41
13C DNP of biomolecules dissolved in water — •Björn C. Dollmann1, Konstantin Gruß1, Lasse Jagschies1, Laura M. Schreiber2, Hans W. Spiess1, Dariush Hinderberger1, and Kerstin Münnemann1 — 1Max Planck Institute for Polymer Research, Mainz, Germany — 2Section of Medical Physics, Mainz University Medical Center, Mainz, Germany
Nuclear magnetic resonance (NMR) and related techniques have become indispensable tools with innumerable applications in physics, chemistry, biology and medicine. One of the main obstacles in NMR is its notorious lack of sensitivity, which is due to the low equilibrium polarization of nuclear spins at ambient temperature. This disadvantage becomes obvious if low γ nuclei are employed for NMR spectroscopy and MR imaging or when small sample volumes should be investigated. However, this obstacle could be overcome by in vitro hyperpolarization of molecules via dynamic nuclear polarization (DNP). One major issue of this approach is the limited lifetime of the hyperpolarized state, which restricts the application and detection of the hyperpolarized molecules to roughly three times T1. Therefore a lot of effort is put into the hyperpolarization of biomolecules with long spin lattice relaxation times. In this work, we present direct Overhauser-type DNP enhancement of 13C in solution at ambient temperatures. For a 5 µ l sample of 10 M 13C-enriched urea with 40 mM TEMPOL dissolved in water we measured a 13C signal enhancement of -335 in a commercial X-band electromagnet.