Regensburg 2010 – wissenschaftliches Programm
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SYMR: Symposium Nuclear Magnetic Resonance: from Applications in Condensed-Matter Physics to New Frontiers
SYMR 7: Biopolymers and Biomaterials
SYMR 7.9: Vortrag
Mittwoch, 24. März 2010, 16:30–16:45, H37
Protein-resistant polymer coatings based on surface-adsorbed poly(aminoethyl methacrylate)/poly(ethylene glycol) copolymers — •Leonid Ionov1, Alla Synytska1, Elisabeth Kaul1, Stefan Diez2, and Manfred Stamm1 — 1Leibniz-Institut fuer Polymerforschung Dresden e.V., Dresden, Germany — 2Max-Planck-Institute of Molecular Cell Biology and Genetics, Dresden, Germany
We report on the protein-resistant properties of glass substrates coated with novel copolymers of 2-aminoethyl methacrylate hydrochloride and poly(ethylene glycol) methyl ether methacrylate (AEM-PEG). In comparison to currently available protein-blocking polymer systems, such as poly-L-lysine - poly(ethylene glycol), silane-based poly(ethylene glycol) and poly(ethylene glycol) brushes prepared by surface-initiated polymerization, the proposed AEM-PEG offers the combined advantages of low cost, simplicity of use and applicability in aqueous solutions. We demonstrate the capability of AEM-PEG to block the surface binding of globular proteins (tubulin), their assemblies (microtubules) and functional motor proteins (kinesin-1). Moreover, we demonstrate the applicability of AEM-PEG for surface patterning of proteins in microfluidic devices.