Regensburg 2013 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Posters: Imaging

BP 10.10: Poster

Montag, 11. März 2013, 17:30–19:30, Poster B2

Chasing the next level in molecular sensitivity — •Henning Hachmeister, Michael Stührenberg, Lena Nolte, Christian Pilger, Gerd Wiebusch, and Thomas Huser — Biomolecular Photonics, Universität Bielefeld, Germany

Fluorescence microscopy is a well-established, molecularly specific imaging technique in the life sciences which continues to gain popularity due to the ongoing development of novel fluorescent probes and superresolution imaging techniques. The use of fluorescent probes, however, is potentially detrimental to biological activity and requires careful controls. Rapid photobleaching and the toxicity of many of these probes greatly limit their use in physiological studies of cells.

A new way to image the structure of cells is the use of vibrational spectroscopy as a microscopic imaging technique. In vibrational spectroscopy, vibrations of molecular bonds inside a molecule are excited using laser beams. Within the last decade the nonlinear optical imaging of molecular modes by coherent anti-Stokes Raman scattering (CARS) has been developed to the point where it can now be routinely use to image e.g. lipid structures within cells within the need for fluorescent probes.

Here, we demonstrate a versatile, new technique called doubly-resonant CARS (DR-CARS) which can enhance weak CARS signals by up to 1000x to enable the detection and analysis of other molecular bonds besides aliphatic C-H groups.