Regensburg 2013 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Posters: Imaging

BP 10.4: Poster

Montag, 11. März 2013, 17:30–19:30, Poster B2

PyCorrFit - a versatile tool for FCS data analysis — •Paul Müller — Biotechnology Center of the TU Dresden, Germany

We present the general-purpose fluorescence correlation spectroscopy (FCS) evaluation software PyCorrFit. PyCorrFit supports various commercially available FCS setups (e.g. ConfoCor3, Zeiss), and offers several built-in model functions, exploiting a wide range of applications. The program comes with several features for data manipulation, permits the import of external model functions that do not require prior programming skills, and can be expanded to fit the needs of the more versed user. We demonstrate the implementation of PyCorrFit in current research, such as diffusional measurements in lipid monolayers or protein affinity assays in free solution. PyCorrFit is free, open-source and available at http://fcstools.dyndns.org/pycorrfit.