Regensburg 2013 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Posters: Imaging

BP 10.5: Poster

Montag, 11. März 2013, 17:30–19:30, Poster B2

SOFI of GABA-B neurotransmitter receptors in hippocampal neurons elucidates intracellular receptor trafficking and assembly — •Anja Huss¹, Omar Ramírez², Felipe Santibáñez², Andrés Couve², Steffen Härtel², and Jörg Enderlein¹ — ¹III. Institute of Physics: Biophysics, Georg-August-University Göttingen, Germany — ²Institute of Biomedical Sciences, Faculty of Medicine and Nucleus of Neural Morphogenesis (NEMO), Universidad de Chile, Santiago, Chile

The synaptic efficacy of neurons depends on the availability of neurotransmitter receptors and is controlled by their intracellular trafficking routes. GABA-B receptors (GABA-BRs) are heteromeric proteins found at the plasma membrane of dendritic postsynaptic sites. Detailed insights of trafficking routes and thus the assembly of the subunits are still missing.

To address this question we have studied the distribution and co-localization of the GABA-BR subunits in the plasma membrane and in the intracellular compartments in hippocampal neurons with dual-color, 3D Stochastic Optical Fluctuation Imaging (SOFI). SOFI is a fluorescence imaging modality which yields super-resolved spatial resolution, 3D-sectioning and high image contrast.