Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 13: Imaging
BP 13.5: Vortrag
Dienstag, 12. März 2013, 10:45–11:00, H44
Subsurface Imaging of Cells Using Atomic Force Acoustic Microscopy at GHz Frequencies — •Matthias Büchsenschütz-Göbeler1, Jan Rother3, Andreas Janshoff3, Walter Arnold2, and Konrad Samwer1 — 1I. Physikalisches Institut, Universität Göttingen — 2Department of Material Science and Materials Technology, Saarland University — 3Institut für Physikalische Chemie, Universität Göttingen
We describe a technique to image subsurface structures in living biological cells (e.g. Madin Darbin canine kidney cells, type II) using Atomic Force Acoustic Microscopy operating at 1 GHz. The cells are insonified with 1 GHz ultrasonic waves which are amplitude modulated at a fraction or multiple frequency of cantilever contact-resonance [1]. The transmitted signals are demodulated by the nonlinear tip-surface interaction, enabling one to image the inner structure of the cell based on their ultrasonic scattering power. The latter one is determined by the ultrasonic frequency, the acoustic mismatch between the elastic properties of the host material (cytoplasm) and the subsurface objects to be visualized (e.g. nucleus), by their geometry and by diffraction effects. Interference fringes can be seen in both the amplitude and phase images. Besides images, we will present an interpretation of the contrast mechanism for imaging. Financial support by the DFG SFB 937 is thankfully acknowledged.
[1] Imaging of Subsurface Structures Using Atomic Force Acoustic Microscopy at GHz Frequencies, S. Hu and C. Su, and W. Arnold, J. Applied Phys. 109, 084324 (2011)