Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Biomaterials and Biopolymers II (joint with CPP)
BP 17.6: Vortrag
Mittwoch, 13. März 2013, 11:00–11:15, H43
Diffusion and Adsorption of Proteins in Mesoporous Environments — •Sebastian Mörz1 and Patrick Huber2 — 1Experimental Physics, Saarland University — 2Materials Physics and Technology, Hamburg University of Technology
In the recent years, several studies discussed the encapsulation of biomolecules in mesopourous materials and its potential applications in e.g. protein chromatography or as novel means of controlled drug release. Both the diffusion of biomolecules under such confinement and the interaction with the surface of the host material are crucial to these applications.
In this study, we examine the adsorption of bovine heart cytochrome c onto the pore surface of the porous silica material SBA-15. Comparison between the folded and unfolded state of this protein allows us to separate the contributions from the different interaction mechanisms involved i.e. coulombic and hydrophobic interaction. Furthermore, we attempt a qualitative validation of the Stokes-Einstein equation for the diffusion of proteins in a porous anodized aluminum oxide membrane and its applicability for protein separation.