Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 21: Membranes and Vesicles II
BP 21.4: Vortrag
Mittwoch, 13. März 2013, 16:00–16:15, H46
Non-Equilibrium Dynamics in Lipid Multilayers - Time Resolved X-Ray Diffraction at In-House and Synchrotron Sources — •Tobias Reusch1, Markus Osterhoff1, Christina Bömer1, Florian Schülein2, Andre Beerlink3, Dong-Du Mai1, Achim Wixforth2, and Tim Salditt1 — 1Institut für Röntgenphysik, Georg-August Universität Göttingen, Germany — 2Lehrstuhl für Experimentalphysik, Universität Augsburg, Germany — 3Deutsches Elektronen Synchrotron, Hamburg, Germany
Collective excitations in biological membranes are of major importance for the understanding of numerous processes in living organisms. Studies of equilibrium properties revealed a rich spectrum of dynamics from the ps to the µ s time scale. Out-of-equilibrium dynamics as driven by external forces, i.e. due to active membrane proteins, have remained largely unexplored.
We report on time resolved X-ray scattering experiments at in-house fs Cu−Kα and synchrotron (ID09B/ESRF, P08/PETRAIII) sources, using optical and surface acoustic wave (SAW) excitation mechanisms. Importantly, we were able to record specular and diffuse scattering at a signal level compatible with a full lineshape analysis. For SAW stimulated lipid bilayers the first time resolved electron density profile could be reconstructed at molecular resolution. A clear out of equilibrium behaviour can be observed for Texas-Red labeled lipid multilayers in case of short pulse optical excitation. These results are compared to theoretical predictions for non-equlibrium phenomena, possible implications for fluorescent microscopy experiments are discussed.