Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 21: Membranes and Vesicles II
BP 21.5: Vortrag
Mittwoch, 13. März 2013, 16:15–16:30, H46
Physical vapor deposition (PVD) of 1,2-dipalmitoyl-sn-3-phosphoglycerocholine (DPPC) and membrane formation on SiO2/Si(100) substrate — •Ulrich G. Volkmann1, María J. Retamal1, Carmen González1, Mauricio Sarabia1, Marcelo Cisternas1, Michael Kappl2, and Tomás Corrales2 — 1Surface Lab, Facultad de Fisica, Pontificia Universidad Catolica de Chile, Chile — 2Max Planck Institute for Polymer Research, Mainz, Germany
Phospholipids have been currently of great interest in nanotechnology and applied sciences. One of the most widely used and interesting phospholipids is the surfactant 1,2-dipalmitoyl-sn-3-phosphoglycerocholine (DPPC). Different techniques have been developed in order to assure the complete formation of bilayers or multilayers and particularly to improve thickness accuracy. Between the most popular are spin-coating, dip-coating and Langmuir-Blodgett. In this work we explore the possibility to achieve controlled deposition of a DPPC bio-membrane, combining physical vapor deposition (PVD) on SiO2/Si(100) substrates under high vacuum conditions with high resolution Ellipsometry for very precise thickness control. The well known phase transitions of DPPC layers were analyzed during temperature cycles with Imaging Ellipsometry, AFM and Raman. Previous to and after the application of temperature cycles the film was inspected with SEM. We suggest a method for prolonged membrane humidification by deposition of an ultra thin porous poliglusam interlayer.
M.J. R. and C. G. acknowledge CONICYT and VRI Nr. 10/2010 (PUC), resp. Work supported by FONDECYT grant Nr. 1100882.