Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 24: Posters: Physics of Cells
BP 24.8: Poster
Mittwoch, 13. März 2013, 17:30–19:30, Poster C
Photo-induced switchable cell adhesion on nanostructured surfaces — •Laith Kadem1, Qian Li1, Michelle Holz2, Rainer Herges2, and Christine Selhuber-Unkel1 — 1Christian-Albrechts-University Kiel, Institute for Materials Science — 2Christian-Albrechts-University Kiel, Otto Diels-Institute of Organic Chemistry
Cell adhesion (CA) relies on the specific binding of transmembrane proteins to their extracellular ligands. The spacing between individual integrin binding sites in mammalian RGD-integrin CA system controls CA forces and reinforcement as well as cell elasticity. We aim to develop nanostructured surfaces where light-driven switchable CA is feasible. Using Diblock Copolymer Micelle Nanolithography, these nanostructures are introduced on the surface as nanometer-sized monodispersed gold particles ordered in a quasi-hexagonal pattern. The spacing between gold dots can be varied from 20 to 200 nm with nanometer precision. Moreover, we can apply this technique to surfaces with a structured microtopography. Regular microtopographies on surfaces can be obtained with photolithography followed by an etching step. Subsequently, we create patterns of the gold nanodots within the micro-domains. With this protocol, we are able to generate different spacings of gold dots on one single substrate in a single step. The gold dots are functionalized with photoswitchable azobenzene molecules incorporated with RGD peptides in order to mediate specific CA to surfaces through integrins. Using the photoswitching properties of azobenzenes, we aim at switching CA in a spatially and temporally defined fashion.