Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 25: Posters: Cytoskeleton
BP 25.16: Poster
Mittwoch, 13. März 2013, 17:30–19:30, Poster C
Correlating the signaling cascade with movement in Dictyostelium discoideum — •Christoph Blum1,2, Vladimir Zykov1,2, Kaumudi Prabhakara1,3, Marco Tarantola1,4, and Eberhard Bodenschatz1,2,3 — 1Max-Planck-Institu für Dynamik und Selbstorganisation, Göttingen, Germany — 2Georg-August-Universität, Institut für Nichtlineare Dynamik, Göttingen, Germany — 3Cornell University, Ithaca, USA — 4University of California, San Diego, USA
Actin cytoskeletal dynamics provide the fundamental basis of eukaryotic cell motility. The cross-linked actin network at the front of a cell pushes the leading edge of the membrane towards the source of attractant. It is our aim to provide a quantitative understanding of the spatio-temporal dynamics of the actin cytoskeleton within the actin cortex.
We have developed experimental methods to address single Dictyostelium discoideum cells with well-controlled mechanical and chemical stimuli. Our experimental techniques are based on microfluidic devices, such as flattening device and micromixer, and fluorescence microscopy (Confocal, TIRF).
Here we present correlations of an important protein in the signaling cascade(Ras-GTP) with the actin polymerization as well as correlations of Ras-GTP localization with pseudopod dynamics. The localization is visualized by the Ras binding domain(RBD-GFP). The pseudopod formation is analyzed by curvature maps. The polymerization of actin is shown by the filametous actin marker LimE.