Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 27: Biotechnology and Bioengineering
BP 27.3: Vortrag
Donnerstag, 14. März 2013, 10:15–10:30, H43
Diffusion and freezing transition of rod-like DNA origami on freestanding lipid membranes — •Eugene P. Petrov1,2, Aleksander Czogalla2, Dominik J. Kauert3, Ralf Seidel3, and Petra Schwille1,2 — 1Max Planck Institute of Biochemistry, Department of Cellular and Molecular Biophysics, 82152 Martinsried, Germany — 2Biophysics, BIOTEC, Technische Universität Dresden, 01307 Dresden, Germany — 3DNA Motors, BIOTEC, Technische Universität Dresden, 01307 Dresden, Germany
During the last decade, DNA origami has become a powerful tool in research at the nanoscale. The relative ease of constructing functionalized DNA origami structures of a defined shape allows for their applications in membrane biophysics. Recently, we have constructed stiff rod-like DNA origami structures consisting of six DNA helixes, which were functionalized with hydrophobic membrane-binding anchors and fluorescently labeled at defined positions [1]. Selective fluorescent labeling allowed us to determine the translational and rotational diffusion coefficients of the DNA origami rods on lipid membranes by fluorescence correlation spectroscopy, which were found to be in a good agreement with the hydrodynamics-based theory of membrane diffusion. Further, we studied the effect of the surface density of membrane-bound origami structures on their Brownian motion. Our results indicate that the 2D membrane hydrodynamics plays an important role in determining the onset of the freezing transition for membrane-bound nanorods.
[1] A. Czogalla, E. P. Petrov, D. J. Kauert, V. Uzunova, Y. Zhang, R. Seidel, and P. Schwille, Faraday Discuss. (2013) in press