Regensburg 2013 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 8: Posters: Proteins
BP 8.7: Poster
Montag, 11. März 2013, 17:30–19:30, Poster B2
Hydrophobin adsorption to the air/water interface: Unusual adsorption kinetics and their origin — •Jonas Raphael Heppe1, Sebastian Backes1, Hendrik Hähl1,2, and Karin Jacobs1 — 1Saarland University, Experimental Physics, 66041 Saarbrücken, Germany — 2University of Zurich, Institute of Physical Chemistry, 8057 Zürich, Switzerland
Adsorption of proteins to the interface between water and other liquids (e.g. oil) or air is of major technological interest. Adsorbed proteins stabilize this interface and hence serve as emulsifying or foaming agent. In food industry, this characteristic is used for products such as milk, beer, or coffee. To optimize production sequences and to be able to give predictions, a deeper understanding of the competitive processes leading to the final adsorbate is necessary. Hydrophobins are a class of proteins that have emerged to have a high technological potential and may also serve as ideal model candidates. Produced by filamentous fungi, hydrophobins are extremely conformationally stable, particularly amphiphilic, highly surface active, and form ordered layers at the water surface [1,2]. We studied wild types and specifically designed mutants of hydrophobins featuring different properties, sizes, and forms. To access the processes involved in the adsorption, we used ellipsometry to record in situ the adsorbed amount at different ambient and solution conditions. Our results reveal adsorption kinetics that significantly differ from that of other systems and cannot be explained by conventional theoretical models. [1] S. Varjonen et al., Soft Matter 7 (2011) 2402 [2] T. Hakala et al., RSC Advances 2 (2012) 9867