Regensburg 2013 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 48: (Hydro)gels and Elastomers
CPP 48.6: Vortrag
Freitag, 15. März 2013, 11:00–11:15, H39
Immobilization of water soluble peroxidase within p- NIPAM microgel particles for the usage in organic solvents — •Kornelia Gawlitza1, Radostina Georgieva2, and Regine von Klitzing1 — 1Stranski-Laboratorium, Institut für Chemie, Technische Universität Berlin — 2Charité Universitätsmedizin Berlin, Department of Enzyme Technology
Enzymes are of high research interest in the field of catalysis. To reach a high stability in the presence of organic solvents, enzymes can be immobilized within a polymer matrix. Microgel particles made of poly- N-isopropylacrylamide (p-NIPAM) are promising as polymer matrix due to their reversible shrinking above the volume phase transition temperature (VPTT). In this study, large p-NIPAM microgels with a cross-linker content of 0.25% were synthesized via surfactant free emulsion polymerization using a temperature ramp[1]. The polymer particles were characterized by Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM). Afterwards, water soluble peroxidase was immobilized within the polymer network using a solvent exchange from polar to organic solvents[2],[3]. The immobilized system shows an enhanced activity in isopropanol and the localization of the enzymes has been proven by confocal laser scanning microscopy (CLSM). [1] Meng, Z.; Smith, M. H.; Lyon, L. A. Colloid. Polym. Sci. 2009, 287, 277. [2] Gawlitza, K.; Wu, C.; Georgieva, R.; Wang, D.; Ansorge- Schumacher, M.-B.; Klitzing, v. R. Phys. Chem. Chem. Phys. 2012, 14, 9594. [3] Gawlitza, K.; Wu, C.; Georgieva, R.; Ansorge-Schumacher, M.-B.; Klitzing, v. R. Z. Phys. Chem. 2012, 226, 749