Dresden 2014 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 13: Posters: DNA/RNA and related enzymes

BP 13.8: Poster

Dienstag, 1. April 2014, 09:30–12:30, P1

Experiments of DNA-Ligand-Complexes with Optical and Magnetic Tweezers — •Ying Wang¹, Susan Haji Samo¹, Andy Sischka¹, Helene Schellenberg¹, Katja Tönsing¹, Thomas Jany², Thorsten Glaser², and Dario Anselmetti¹ — ¹Experimental Biophysics and Applied Nanoscience, Bielefeld University, 33615 Bielefeld, Germany — ²Lehrstuhl für Anorganische Chemie I, Bielefeld University, 33615 Bielefeld, Germany

We investigated the specific binding of Co-, Cu- and Ni-dinuclear metal complexes (DNMCs) to dsDNA with optical (OT) and magnetic tweezers (MT). DNMCs bind to the DNA backbone, hydrolyse phosphate esters (cutting functionality) and thus prevent the particular DNA segment from being replicated or transcribed. In the OT stretching experiments, we have observed force peaks, suggesting knot or coil formation of DNA entanglements, since the aromatic groups of neighbouring DNMCs can interact with each other via π-stacking. Detecting these complexations have proved the binding of DNMCs to DNA. In twisting experiments with MT, we have evidenced that 200µM Cu-DNMC shows cutting functionality. Because of the created nicks, the DNA molecule can no further be twisted. Combining with molecular recognition techniques DNMCs could be utilized on medical applications as a possible drug dealing with cancers in the future.