Regensburg 2016 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 79: Graphene III: Electronic Properties
O 79.9: Vortrag
Donnerstag, 10. März 2016, 12:30–12:45, S053
Extracellular stimulation of electrogenic cells using graphene devices — •Karolina Stoiber1, Martin Lottner1, Markus Manz1, Simon Drieschner1, Benno Blaschke1, Martin Stutzmann1, and José A. Garrido1,2 — 1Walter Schottky Institut, Technische Universität München, Garching, Germany — 2Catalan Institute of Nanoscience and Nanotechnology, Barcelona, Spain
Graphene is a highly conductive, chemically stable, flexible and biocompatible material. Therefore, Graphene microelectrode arrays (GMEAs) are a promising bio-sensing and cell stimulation platform in neural implants. In this work, the electrical stimulation of HEK293 and HL1 cells through extracellular voltage trains by GMEAs is presented. Further, the investigation of the biocompatibility of graphene foam and its capability for extracellular stimulation is discussed.
GMEAs with circular electrodes were fabricated using standard photo-lithography techniques. For the characterization of the devices Raman-spectroscopy and cyclic voltammetry were used. The cell membrane potential of HEK293 cells was monitored during extracellular stimulation via patch-clamping. HL1 cells, a cardiomyocyte-like cell line, were dyed with a calcium-sensitive fluorophore and the modulation of their firing frequency upon stimulation was recorded.
Graphene foam devices were fabricated as previously described. The devices were characterized electrochemically and their capability for extracellular stimulation of HL1 cells tested.