Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 24: Posters - Protein Structure and Dynamics

BP 24.6: Poster

Dienstag, 21. März 2017, 14:00–16:00, P1A

Fast liquid DSC - a novel tool to study protein solutions — •Jana Rüdel, Patrizia Kruse, and Maria Ott — Institute of Physics, Martin-Luther-University Halle-Wittenberg, 06120 Halle, Germany

Fast scanning calorimetry with heating rates up to 10000 K/s allows to study rapid non-equilibrium processes which remain invisible in standard DSC experiments. The available sensor chips, however, which were successfully used in polymer science [1] restricted the use to dry or highly viscious samples. If this technique could be extended to liquid samples, fast scanning calorimetry would give a unique access to study the time dependencies of processes in biology.

We extensively tested novel, recently introduced liquid Flash DSC sensors [2] by studying aqueous protein solutions with scanning rates up to 500K/s. We will illustrate the potential of using fast scanning calorimetry and discuss current challenges and limitations.

[1] V. Mathot et al., Thermochim Acta 522 (2011), 36-45

[2] R. Splinter et al., Thermochim Acta 603 (2015), 162-171

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