Dresden 2017 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 48: Membranes and Vesicles II

BP 48.5: Vortrag

Donnerstag, 23. März 2017, 10:45–11:00, HÜL 386

Receptor distribution in supported lipid bilayer upon binding of norovirus like particle — •Nagma Parveen¹, Daniel Midtvedt¹, Vladimir Zhdanov¹, Gustaf Rydell², Vesa Hytonen³, and Fredrik Höök¹ — ¹Department of Physics, Chalmers University of Technology, Gothenburg, Sweden — ²Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden — ³BioMediTech, University of Tampere, Biokatu 6, FI-33520 Tampere, Finland

Prior to internalization and infection, virions first bind to specific receptors present on the external lipid membrane of their host cells. This process is typically dynamic and multivalent, and known to influence the receptor distribution on the cell membrane and the shape of membranes, factors that are believed to be also crucial during the internalization process. To explore this, we have used fluorescently labeled histidine-tagged virus like particles (VLP) of norovirus and followed their binding to histo-blood group antigens (HBGAs) embedded in cell-membrane mimic, i.e. supported lipid bilayer (SLB). These HBGAs, e.g. Btype1 and Htype1 are known to be natural receptors of norovirus. Interestingly, we found that in case of Btype1 the VLPs bind in small clusters (1-2 μm) whereas the binding is homogeneous to Htype1 indicating that Btype1 forms clusters in SLB upon VLP binding. The kinetics of VLP binding and cluster growth is detected using time-lapse total internal fluorescence microscopy. The cluster formation is further supported by a competitive binding assay using inhibitious lectin.