Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 10: Postersession II
BP 10.41: Poster
Montag, 12. März 2018, 17:30–19:30, Poster C
3D-environments shape T-cell motility and cell-cell contacts during HIV-1 infection — Andrea Imle1, •Nikolas Schnellbächer2,3, Peter Kumberger3, Jana Fehr3, Paola Carillo-Bustamante3, Frederik Graw3, Ulrich Schwarz2,3, and Oliver Fackler1 — 1Department of Infectious Diseases, Integrative Virology, University Hospital Heidelberg — 2Institute for Theoretical Physics, Heidelberg University — 3BioQuant, Heidelberg University
The spread of HIV-1 can progress either through cell-free infection or through direct cell-cell contacts between immune cells. The latter mode is assumed to be more efficient, but it remains elusive which strategy is favored under different conditions and how tissue structure might change the contribution of each mode of infection to viral spread. To address this problem, we study HIV-1 infection dynamics of primary T-lymphocytes in tissue-like 3D environments (collagen matrices of different densities). Based on single cell tracking data, we developed a quantitative analysis to study how different 3D environments influence cell migration and shape the kinetics of cell-cell contacts. This information is then used to parameterize a Cellular Potts Model (CPM). Applying this CPM in combination with population dynamics models to the infection dynamics data, we infer kinetic parameters of HIV-1 spread under different environmental conditions. Together, our work provides mechanistic and quantitative insight to understand how 3D environments shape HIV-1 spread.