Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 10: Postersession II
BP 10.59: Poster
Montag, 12. März 2018, 17:30–19:30, Poster C
Interkinetic nuclear migration as a stochastic process in the zebrafish retina — Afnan Azizi1, •Anne Herrmann2, Salvador J. R. P. Buse1, Yinan Wan3, Philipp J. Keller3, Raymond E. Goldstein2, and William A. Harris1 — 1Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom — 2Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Cambridge, United Kingdom — 3Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA, USA
Interkinetic nuclear migration (IKNM), a movement of nuclei between the apical and basal surfaces of proliferating cells in developing epithelia, was first observed more than 80 years ago. Since, IKNM has been studied in multiple organisms but despite these efforts many questions about the role and mechanism of this process remain unsolved. In earlier studies, only single or sparsely labelled nuclei in an otherwise unlabelled tissue were imaged. Here, we present data from light sheet microscopy on wholly labelled retinas, where the movement of all nuclei in a tissue section can be followed with high temporal resolution. This approach enables us to study the movements of individual nuclei as well as their collective behaviour in a systematic fashion. Our data, in combination with mathematical models, support the hypothesis of IKNM as a stochastic process. These results present IKNM as a possible precursor for the observed stochasticity in progenitor differentiation and have important implications for understanding the organisation of developing vertebrate tissues.