Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.13: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Synergetic Effects of a Cationic Surfactant and Alcohol in Antibacterial Function — •Judith Thoma1, Wasim Abuillan2, Ippei Furikado3, Shigeto Inoue3, Thomas Gutsmann4, Klaus Brandenburg4, Oleg Konovalov5, and Motomu Tanaka1,6 — 1Institute for Physical Chemistry, University of Heidelberg, Germany — 2Department of Fundamental Engineering, University of Tokyo, Japan — 3Analytical Science Research, Kao Corporation, Tokyo, Japan — 4Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Germany — 5European Synchrotron Radiation Facility (ESRF), Grenoble, France — 6Institute for Integrated Cell-Material Science, Kyoto University, Japan
The outer membrane of Gram negative bacteria displays a dense layer of lipopolysaccharides (LPSs) that protects the bacteria against environmental changes. Previously, Kao Co. (Japan) demonstrated significant improvement in anti-bacterial activity of cationic surfactants in coexistence with benzyl alcohol (BA). Simultaneous measurements of X-ray reflectivity (XRR) and grazing incidence X-ray fluorescence (GIXF) at the interface between sanitary agents and model bacterial surfaces based on LPSs determined how the fine structures and ion density profiles in the proximity of the interface is altered by cationic surfactants and benzyl alcohol. Focus is put on the localization of Ca2+ ions, which were proved to play a vital role in rejecting cationic antibacterial peptides from LPS monolayers. A significant enhancement of interactions between cationic surfactants and LPSs could be demonstrated by the addition of BA even in the presence of Ca2+ ions.