Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.16: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Study of elastic modulus of phospholipid bilayers — •MJ Retamal1, R Catalan2, M Cisternas2, N Moraga2, D Diaz2, TP Corrales3, M Busch4, P Huber4, M Soto-Arriaza1, and UG Volkmann2 — 1Faculty of Chemistry and CIEN-UC, P. Univ Catolica de Chile, Santiago, Chile — 2Institute of Physics and CIEN-UC, P. Univ Catolica de Chile, Santiago, Chile — 3Department of Physics, UTF Sta Maria, Valparaiso, Chile — 4TUHH, Hamburg, Germany
Study of artificial phospholipid membranes (PMs) on plane substrates has become relevant to contribute in the field of Bionanotechnology. In this work, we analyse the temperature dependence of Youngs modulus (YM) of several PMs (DPPC, DMPC and DSPC) performing Atomic Force Microscopy (AFM) and Surface Force Spectroscopy (SFS) measurements. Phospholipids were deposited in high vacuum on silicon substrates by physical vapour deposition (PVD). Using Raman Spectroscopy, we confirmed that the chemical structure of our phospholipids remains unchanged after PVD. AFM measurements in liquid confirm the self-assembly of the phospholipid bilayer. YM measurements obtained by SFS show the main transitions of the phospholipid bilayers. With this we have shown that PMs can be made by PVD in high vacuum, preserving their structure and mechanical properties after proper hydration. This study opens new pathways to assemble phospholipid mixtures by PVD. Acknowledgement: Postdoctoral grant FONDECYT 3160803 (MJR), FONDECYT grant #1141105 (UGV)and #1171047 (MSA), FONDECYT INICIACION grant #11160664 (TPC), CONICYT Fellowships (RC and MC) and CONICYT-PIA ACT 1409.