Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.21: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Lentiviral infection leads to blue fluorescent labeling of cancer cells — •Lorena Hentschel1, Maja Strugacevac1, Constanze Wiek2, Julia Kristin2, Marcel Glaas2, Jörg Schipper2, and Mathias Getzlaff1 — 1Institute of Applied Physics, University of Düsseldorf, Universitätsstr. 1, 40225 Düsseldorf, Germany — 2Düsseldorf University Hospital, Department of Otorhinolaryngology, Moorenstraße 5, 40225 Düsseldorf
Previous studies in biophysics show that the behavior and properties of cells depend on their physiological environment, weather it is the coating or their coexistence with other cells. A simultaneous investigation of different cell types, such as in a human body, is indispensable. To do so, a specific labeling is necessary in order to distinguish one cell type from the other unambiguously.
Our group's research deals with the morphological differences between squamous cell carcinoma cells and non-tumor dysplastic oral keratinocytes of the head-neck area. Using a laser scanning fluorescence microscope, e.g., cell membrane and mitochondria can be investigated in detail. In order to label the carcinoma cells, a lentiviral vector is applied which results in translating a blue fluorescent protein. This method allows the research of two co-cultivated different cell types under the same experimental conditions and possible change of properties due to interaction.
As mitochondria play a huge role in the behavior of cancer cells, we focus on the investigation of their differences in the two observed cell types. Our latest results are presented in this contribution.