Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.23: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Low Energy Electron Holography as a tool for imaging single proteins at high resolution — •Hannah Ochner1, Sven Szilagyi1, Wolfgang Stiepany1, Peter Andler1, Marko Memmler1, Sabine Abb1, Stephan Rauschenbach1,2, and Klaus Kern1,3 — 1Max-Planck-Institut für Festkörperforschung, 70569 Stuttgart — 2Chemistry Research Laboratory Department of Chemistry, University of Oxford — 3École polytechnique fédérale de Lausanne, CH-1015 Lausanne
Protein function is intimately linked to the protein’s native 3D folding, hence determining these structures is of tremendous importance. Low Energy Electron Holography (LEEH) [1], pioneered by Fink and colleagues, is an elegant imaging method using coherent low energy electrons (50-200eV)[2] avoiding radiation damage and hence allowing for single molecules investigations [3]. Because holograms contain information regarding both amplitude and phase of the object wave field, a full 3D reconstruction can in principle be obtained by numerical reconstruction of experimentally acquired holograms. Thus, unlike in other structure determination methods such as Cryo-EM and XRD at FELs, averaging is not required. The poster gives an overview of the experimental technique and a new setup, along with the theoretical background and preliminary results.
[1] Phy. Rev. Lett, 1990, 65(10), 1204-1206.
[2] Phys. Scr., 1988, 38, 260
[3] PNAS 114, 1474-1479 (2017)