Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 15: Postersession III

BP 15.25: Poster

Dienstag, 13. März 2018, 14:00–16:00, Poster B

Distance sensing using Metal Induced Energy Transfer (MIET) — •Fabian Port and Kay-E. Gottschalk — Institute of Experimental Physics, Ulm University, Germany

In the last few decades the correlation between cell mechanics and different physiological or pathophysiological conditions, like stem cell differentiation [1] or cancer [2], has been a growing aspect of biophysical research. To understand the underlying mechano-chemical feedback cycles, it is important to understand the mechanical properties of cells under varying conditions. Cell mechanics is to a large extent determined by the cells’ cytoskeleton. For a detailed analysis of the cytoskeletal structures, a method to measure small distances in cells is needed. A technique which meets this challenge is Metal Induced Energy Transfer (MIET) [3]. Here we show a first analysis of the distance between vimentin and the underlying surface in different cell lines and demonstrate the usefulness of MIET for analyzing cytoskeletal structures close to the basal membrane.

References:

[1] Suresh, S., Spatz, J., Mills, J. P., Micoulet, A., Dao, M., Lim, C. T.,and Seufferlein, T. (2005). Connections between single-cell biomechanics and human disease states: gastrointestinal cancer and malaria. Acta Biomaterialia, 1(1), 15-30.

[2] Sokolov, I. (2007). Atomic force microscopy in cancer cell research. Cancer Nanotechnology, 1-17.

[3] Chizhik, A. I., Enderlein, J. et al. (2014). Nature Photonics, 1-8. http://doi.org/10.1038/nphoton.2013.345