Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 15: Postersession III

BP 15.32: Poster

Dienstag, 13. März 2018, 14:00–16:00, Poster B

Photonic Force Microscopy (PFM) on cell cultures — •Lilian Weißer, Tobias Neckernuss, Othmar Marti, and Heinrich Hörber — Institute of Experimental Physics, Ulm University

In confocal microscopy a high spatial resolution is obtained by focusing a laser to diffraction limit. Only the focus volume of the laser illuminating the sample is observed. The sample, usually biological cells, is a 100µm thick glass sample chamber. This chamber is located between a 100x oil immersion objective and condenser. In such a setup the laser focus allows also the manipulation of a nanoparticle using optical forces. An interference detection scheme using a quadrant photodiode enables position detection of nanoparticles in the focus with nanometer precision and time resolution of microseconds. Such a setup can be used to characterize interactions of nanoparticles with the environment. Until now this PFM was mainly used for mechanical characterization of the molecular motor Kinesin and so called membrane rafts in living cells. To do experiments directly in cell culture dishes with optically graded thin glass at the bottom, a 100x water immersion objective will be used as a condensor. The PFM can be employed for long time observations on living cells, which can be kept healthy in the dishes for the time of the measurement, even if it lasts for days.