Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.35: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Design and Instrumentation of an Opto-digital Confocal Microscope — •Berk Zengin1, Adnan Kurt3, and Alper Kiraz1,2 — 1Department of Physics, Koc University, Istanbul, Turkey — 2Department of Electrical and Electronics Engineering, Koc University, Istanbul, Turkey — 3Teknofil Limited Company, Istanbul, Turkey
Confocal microscopy has become a vital technique for life sciences due to higher lateral and axial resolution it provides compared to standard epifluorescence microscopy. Improved axial resolution increases the sectioning capability, allowing for the observation of living specimens in three dimensions.
Despite these, accessibility to confocal microscopes did not escalate in proportion to its usage around the globe. Therefore, it was aimed to build an opto-digital confocal microscope, potentially leading to a product which will be affordable for researchers and organizations at a diverse scale.
In this work, we present a home-built confocal microscope setup using commercially available equipment. The design of the setup was realized using a 488 nm laser, an inverted microscope and optical/optomechanical parts. In addition, dual axis galvo scanner was controlled by using a custom built control electronics. Instrumentation was made using a National Instruments DAQ card and LabVIEW based software. For characterization purposes, calibration ruler and CD pattern were successfully imaged, followed by imaging of biological samples such as HeLa cells.