Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.37: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Acoustophoresis: A powerful application in microfluidics for focussing and sorting microparticles — •Toni Schildhauer1, Thomas Henkel2, and J. Michael Köhler1 — 1Technische Universität Ilmenau, FG. Physikalische Chemie/Mikroreaktionstechnik, Prof.-Schmidt-Str. 26, 98693 Ilmenau, Deutschland — 2Leibniz Institut für Photonische Technologien IPHT Jena, AG. Mikrofluidik, Albert-Einstein-Str. 9, 07745 Jena, Deutschland
This work reports the implementation and testing of acoustophoresis into micro structured fluid channels for the purpose of focussing respectively sorting microparticles of different sizes and materials. To get valid images for e.g. flow cytometry cells need to be in the focal plane. Usually particles in microfluidic channels are distributed throughout its whole height, which is a multiple of the particles diameter as well as the range of the focal plane. Acoustophoresis was integrated into a microfluidic chip design of the IPHT Jena. Using micro algae and polystyrene particles in a size range of 10 um it was shown, that all particles can be moved into one plane under the influence of acoustophoretic forces and hence imaged sharply. Furthermore, two new microfluidic chips were designed for particle sorting application by acoustophoresis. With acoustophoresis, sorting by size is achievable up from 1 um upwards. With polystyrene particles in a diameter range from 3 to 25 um, the functioning of the sorting application was confirmed by microscopy imaging and DCS (differential centrifugal sedimentation spectroscopy).