Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.38: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Monitoring activity of stem-cell derived cardiac pacemaker cells by scanning ion conductance microscopy — Lennart Groß1, Julia Jeannine Jung2, •Regina Lange1, Christian Völkner1, Sven Kraft1, Ingo Barke1, Christian Rimmbach2, Gustav Steinhoff2, Robert David2, and Sylvia Speller1 — 1University of Rostock, Institute of Physics, 18059 Rostock, Germany — 2University of Rostock, RTC, 18057 Rostock, Germany
Cell-based sensors and assays are typically used to aid drug design and to monitor water, medium, or air quality. A number of transduction mechanisms are employed, such as ion currents or luminescence. In our case we used scanning ion conductance microscopy (SICM) [1] to observe the live-cell morphology and dynamics of individual sinus nodal cardiomyocytes derived by forward programming from pluripotent stem cells [2]. The membrane displacements and surface morphology have been characterized by SICM in the native state on the pacing sinus nodal cells and while transiently inhibited pacing, respectively. Beating patterns in the range of a few Hertz and of a displacement of about 1 µ m were observed. We noticed an influence of the distance between the nanopipette and the cell surface on the beating behavior. Characteristic features in the temporal spectra are analyzed with regard to the electro-mechanic pacing of the sinus nodal cells. An approach to discriminate possible participation of ion current variation directly from the cell is developed.
[1] C-C Chen et al., Annu Rev Anal Chem 5, 207 (2012)
[2] JJ Jung et al., Stem Cell Rep 2, 592 (2014)