Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.47: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Adjustment of pulsed laser radiation for strob oscopic exp eriments — •Stefan Krüger1, Tobias Löffler1, Maja Strugacevac1, Julia Kristin2, Constanze Wiek1, Jörg Schipper2 und Mathias Getzlaff1 — 1Institute of Applied Physics, University of Düsseldorf — 2Düsseldorf University Hospital, Department of Otorhinolaryngology, Mooren- strasse 5, 40225 Düsseldorf, Germany
This contribution aims to adjust the laser radiation of a fluorescence microscope depending on the oscillating movement of a piezoelectric actuator. Through this a stroboscopic effect can be achieved and the periodic movement of cell parts, depending on the phase of the acoustic stimulation, can be observed. The laser can be switched by TTL-signals, the piezoelectric actuator is controlled using sinus voltages with frequencies from 0,5 to 10 kHz. The piezo-control-signal is translated to TTL and the phase and signal-length is adjusted. By controlling the phase shift between the stimulating wave and the laser pulse we can select which phase of the cell movement we observe. The adjustment of the laser pulse length helps to regulate the output power and time resolution of output signal. The set up and a function test will be presented.