Berlin 2018 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 20: Poster Session I

CPP 20.8: Poster

Montag, 12. März 2018, 17:30–19:30, Poster A

Non-Classical Interactions of Phosphatidylcholine with Mucin for Protection of Intestinal Surfaces: A Microinterferometry Study — •Federico Amadei and Motomu Tanaka — Institute of Physical Chemistry, Heidelberg, Germany

Albeit many studies demonstrated that the accumulation of phospholipids in the intestinal mucosal surfaces is essential for the protection of colon epithelia against pathogenic bacteria, the mechanism of interactions between phospholipids and the surface protein mucin is not well understood. In this study, the significance of phospholipid-mucin interactions was quantified by the combination of an in vitro intestinal surface model and label-free microinterferometry. The model of intestinal surfaces consists of planar lipid membranes deposited on solid substrates that display mucin proteins at defined anchoring densities. Following the characterization of the systems by quartz crystal microbalance and fluorescence staining, the vertical Brownian motion of cell-sized particles on model surfaces was monitored by using RICM. In case of latex particles bearing different surface charges, the calculated potential curvature monotonically increased according to the increase in surface potential, suggesting the dominant role of electrostatic interactions with negatively charged mucin in the potential confinement. However, the particles coated with phospholipids exhibited a clear nonlinearity: the particles coated with zwitter-ionic phosphatidylcholine is most sharply confined on mucin surfaces. Our data suggested the presence of another underlying molecular level interaction that is stronger than electrostatic interactions.