Regensburg 2019 – wissenschaftliches Programm
CPP 25.38: Poster
Dienstag, 2. April 2019, 14:00–16:00, Poster B1
Synthesis of protein-coated gold nanoparticles and investigations on their uptake by diatoms — •Marisa Hoffmann1,2, Nathalie Pytlik1, Roland P. M. Höller2, Christian Kuttner2, Andreas Fery2,3, and Eike Brunner1 — 1Department of Bioananalytical Chemistry, TU Dresden, Germany — 2Leibniz-Institut für Polymerforschung Dresden e.V., Germany — 3Department of Physical Chemistry of Polymeric Materials, TU Dresden, Germany
Because of the inertness and the surface plasmon resonance (SPR) of gold nanoparticles (AuNPs) and the biocompatibility of natural proteins, the combination of both provides a promising system for in vivo surface enhanced Raman spectroscopy (SERS) applications. AuNPs were synthesized by the citrate reduction method and the kinetically controlled seeded growth of citrate-stabilized AuNPs (Bastús 2011). Ligand exchange of the citrate by bovine serum albumin (BSA) was then performed. Gold/gold 3D core/satellite nanoclusters were synthesized by protein assisted self-assembly in solution (Höller 2016). After incubating cells of the diatom species stephanopyxis turris with the mentioned protein-coated AuNPs and gold nanoclusters, respectively, in vivo 3D Raman imaging was carried out. As, for a detectable SERS enhancement, plasmonic nanostructures in the vicinity of the analyte are necessary, the localization of AuNPs can be determined by the position of the SERS signals. That way it was possible to successfully investigate the uptake of AuNPs and gold nanoclusters by the diatom cells. This opens up the way for a new method in prospective in vivo research of algal metabolism.