Regensburg 2019 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 52: Biomaterials and biopolymers I (joint session BP/CPP)
CPP 52.11: Vortrag
Donnerstag, 4. April 2019, 12:15–12:30, H10
Co-survival and competition relationship between bacteria analyzed in millifluidic droplet sequence — •Xinne Zhao1, Larysa Baraban1,2, and Gianaurelio Cuniberti1,2 — 1Institute of Materials Science and Max Bergmann Center of Biomaterials Dresden, TU Dresden, Dresden, Germany — 2Center for advancing electronics Dresden, cfaed, Dresden
Analysis of living systems, e.g. bacterial or cells populations plays significant role in fundamental research of population diversity, and evolution.1 Here, we present an optical detection system, combining the encapsulation of bacteria into numerous emulsion droplets to monitor their long term behavior and their relationship in co-culture environment. The bacteria we choose here are BFP E.coli and YFP E.coli which can express blue fluorescence and yellow fluorescence separately under different light illuminations. By detecting the emission wavelength from different E.coli, we can obtain the information of growth state of each bacteria strain. Compared to the classical cell culture methods, the strategy we use here can avoid the influence of getting sample during bacteria growing, as well achieve real-time and automatic monitoring. In order to find out the co-survival and mutual competition relationship between the two bacteria strains, we plan to get the reference growth curve of individually culturing both strains, co-culture them with different initial cell concentration ratios, add antibiotics, as well as compare their maximum cell concentration and generation time.
[1] R. Illing et al, Biomicrofluidics, 2016, 10, 024115.