Dresden 2020 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Poster III

BP 10.24: Poster

Montag, 16. März 2020, 17:30–19:30, P2/3OG

Visualisation of cytoplasmic flows in epithelial cells by single particle tracking — •Christoph Engwer, Mariam Ristau, and Timo Betz — Institute of Cell Biology, Center for Molecular Biology of Inflammation, Münster, Germany

Correct polarization of epithelial cells is highly important for tissues to perform their respective function. Failure during polarity formation is connected to different diseases such as polycystic kidney disease and malignant cancers. Whereas biochemical signalling, responsible for establishing cellular polarity is well understood, only little is known about the mechanical processes that play a role during polarity formation. In this project, we investigate how cytoplasmic flows may influence cellular polarity or vice versa. As model system, we use spherical cysts made from kidney epithelial cells from domestic dog (MDCK). These cysts form within three to four days when grown in a pseudo-3D environment of extra cellular matrix components. Prior to cyst formation, far-red labelled nanoparticles that serve as tracers for intracellular flows, are ballistically injected into the cells. For the analysis of intracellular flows, we develop a Python based single particle tracking software based using Trackpy. Cysts are imaged over time and particle tracks are obtained from maximum intensity projections around the equatorial plane. To gain average values, cells are registered using labelled nuclei and F-actin. After rotating and projecting each cell into a unit cell, we obtained activity maps of intracellular particle movement.