Dresden 2020 – wissenschaftliches Programm
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BP 10.6: Poster
Montag, 16. März 2020, 17:30–19:30, P2/3OG
Endothelial cell mechanics in inflammation under shear stress inflicted by flow — •Matthias Brandt1, Volker Gerke2, and Timo Betz1 — 1Institut für Zellbiologie, ZMBE, Universität Münster — 2Institut für Medizinische Biochemie, ZMBE, Universität Münster
Localized and tightly controlled leukocyte extravasation is a hallmark of the early inflammatory response. Whereas many cell and protein interactions regulating this process are well described, here we focus on the mechanical role of the endothelium in inflammation prior to leukocyte transmigration. Mimicking inflammation in a human umbilical vein endothelial cell (HUVEC) monolayer via chemical stimulants such as TNF-alpha, traction force and intramonolayer stress microscopy reveal a rapid (30 min) response in traction stress and even more in cell-cell stresses. The majority of cells is found to transfer increasing amounts of internal stress to their neighbors. Intuitively, the quickly altered mechanical state of the endothelium may help in guiding arriving leukocytes. As differences in mechanics for cells under flow as opposed to static conditions have been reported for HUVEC layers in a non-inflammatory state, using a microfluidic setup allowing for the preparation of micropatterned gels inside a flow chamber, we expose the endothelial cells to different levels of shear stress induced by flow. Potential contributions of altered forces among the endothelium to the transmigration process itself will be studied in future experiments via transmigration assays of human neutrophils and a controlled activation of contractility by pharmacologic and optogenetic tools.