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BP: Fachverband Biologische Physik

BP 13: Cell Mechanics I

BP 13.9: Vortrag

Dienstag, 17. März 2020, 12:15–12:30, SCH A251

Estimating biomechanical properties of Head and Neck Squamous Carcinoma Cells (HNSCC) with single-molecular force microscopy — •Hsiao-Ching Tsai¹, Julia Kristin², Jörg Schipper², and Mathias Getzlaff¹ — ¹Institue of Applied Physics, Heinrich-Heine-Universität Düsseldorf — ²Hals-Nasen-Ohren-Klinikum Düsseldorf

AFM is one of the most common approach to access the loading deformation behavior of different soft materials such as tissue or cells. With AFM, a complete data base of biomechanical properties like displacement or deformation could be easily established. The progress of the fundamental biomechanical discovery engages further investigation of living matters. To our knowledge, cancer is one of the most lethal diseases lacking effective treatment. Thus, the development of supplementary or improvement of therapeutic methods lead to an urgent requirement of biomechanics in detail. By means of AFM, cytoskeleton network changing due to the disease progression which is closely associated to the biomechanics is confirmed. In this study, we cultured different head and neck squamous cells from the same area (tongue) at different cancer stages (healthy, benign, cancerous and metastatic) and examined them using a nanoindentation technique. The Hertz model of contact mechanics is adopted to extract the elastic modulus by analyzing the force-indentation curves. Our results present a quantitative model to distinguish the disease conditions of head and neck squamous cells. Three dimensional images of living cells in liquid environment can be visualized simultaneously.