Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster V
BP 17.30: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/1OG
Impact of cell culture age and structural modifications on the mechanical properties of hiPSCs derived cardiomyocytes. — •Emmanuel Manu1, Nithya Shree1, Ricardo Pires1, Stefan Gross2, and Oliver Otto1 — 1Biomechanics, ZIK HIKE, University of Greifswald, Germany — 2DZHK, University of Greifswald, Greifswald, Germany
Cardiomyocytes derived from human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are important biological models in drug screening and in understanding the pathophysiology of many cardiac diseases. Here, using atomic force microscopy, we focus on understanding the mechanical properties of cardiomyocytes as a function of culture age, using Cytochalasin D (CytoD) to promote actin-network depolymerisation. We observed that between the second to the fifteenth day of culture, the Young*s modulus (mean * SEM) of hiPSC-CM increased from 657 * 93 Pa to 1967 * 159 Pa. Exposing hiPSC-CMs to CytoD of varying concentrations, we determined an EC50 of 3.892 *M. Interestingly, the effect of actin depolymerization on the mechanical properties of cells decreases with cell culture age. This indicates that maturation of hiPSC-CMs involves remodelling of the actin network towards increased stability, possibly through the predominant incorporation of actin filaments into sarcomeres. Finally, we compared the elastic modulus of hiPSC-CMs when the cells are characterized in suspension as well as in adherent state. Interestingly, both methods yield elastic modulus values in good agreement.