Dresden 2020 – wissenschaftliches Programm
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BP 18.21: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/2OG
Quantitative Phase Imaging for Studying Cell Migration and Bone Resorption in Periodontitis — •Felix Peter Sanchez Klose, Agnes Dahlstrand Rudin, Karin Christenson, Maria Ransjö, and Johan Bylund — Göteborgs Universitet, Göteborg, Sweden
Periodontitis is a chronic inflammation of the tooth supporting structures caused by a prolonged immune reaction to bacteria resulting in tooth loss if left untreated. As an inflammatory disease, the migration of neutrophils to the site of infections is of interest to study periodontitis. Besides the inflammatory lesions, periodontitis is characterized by loss of the bone structures surrounding the teeth. Imaging of the cell cultures was performed with a quantitative phase imaging (QPI) microscope. We demonstrate that a QPI microscope can be used to measure neutrophil chemotaxis and to perform cell tracking on a cellular level. The system allows for statistical analysis of various chemotactic parameters. In an osteoclast model system, the live imaging allowed for identification of early osteoclast-like cell formation and helped us to pinpoint the ideal culture length for cell quantification. QPI offers a valuable tool to observe neutrophil migration in a label-free system directly in cell cultures. In contrast to conventional methods, QPI can give insights in the neutrophil migration on a single-cell level and it enables analysis of cell movement parameters, e.g., motility. In addition, this method can aid in investigating the processes of cell differentiation, e.g., formation of osteoclasts.