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BP: Fachverband Biologische Physik
BP 20: Poster VIII
BP 20.19: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/4OG
First steps toward reversible protein immobilization on conductive carbon nanomaterials — •Lara Jorde1, Zehao Li2,3, Jacob Piehler2, Changjiang You2, and Carola Meyer1 — 1Department of Physics, University of Osnabrück, Germany — 2Department of Biology, University of Osnabrück, Germany — 3College of Life Science, Beijing University of Chemical Technology, China
The surface immobilization of biomolecules is indispensable for most characterization techniques and therefore a goal in structure analysis. Fundamental understanding of the effects of immobilization on proteins functionalized to these conductive carbon nanomaterials thus enables the development of new types of electronic biosensors. Graphene is used as a calibration material for the CNT functionalization. Here, we achieved the Graphene-functionalization by a pyrene-linker compound binding to Green Fluorescent Protein (GFP) exemplary for a wide range of proteins. Reflectance interference- and total internal reflectance fluorescence spectroscopy reveals a specific and reversible protein binding on graphene with well-defined binding kinetics. Fluorescence quenching caused by graphene is determined by fluorescence lifetime imaging microscopy, confirming a tight yet reversible immobilization of the GFP-fluorophore on graphene. Challenges with regard to the application of this functionalization route and characterization techniques on CNTs are discussed, which have great transport properties for a later analysis of different protein configurations that allow for one-dimensional alignment and high sensitivity of sensing.