Dresden 2020 – wissenschaftliches Programm
Die DPG-Frühjahrstagung in Dresden musste abgesagt werden! Lesen Sie mehr ...
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 20: Poster VIII
BP 20.20: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/4OG
Single molecule localization microscopy in front of a tuned mirror — •Hannah S. Heil1, Marie-Christine Dabauvalle2, Sven Höfling3, Martin Kamp3, Markus Sauer2, and Katrin G. Heinze1 — 1Rudolf Virchow Center, University of Würzburg — 2Biocenter, University of Würzburg — 3Technische Physik, University of Würzburg, Würzburg, Germany
Single-molecule localization microscopy (SMLM) methods have evolved as powerful tools to image cellular structures with virtually molecular resolution. We have demonstrated that higher photon yield at lower background on biocompatible metal-dielectric coatings substantially improves the SMLM performance, and significantly increases the localization precision and, thus, the image resolution (Heil HS et al., Light Sci Appl 7 (99), 2018).
The strength of the approach is that - except for the coated cover glass - no special microscope setup is required. We show that biocompatible metal-dielectric nanostructures fabricated on microscopy coverslips improve the resolution of direct stochastic optical reconstruction microscopy (dSTORM). The enhanced signal-to-noise ratio induced by the metal-dielectric coating sharpens the localization precision, and exceeds Widefield and Total Internal Reflection Fluorescence (TIRF) dSTORM performance without the need for a special TIRF objective lens in a much simpler setup. The resolution improvement is spectrally and spatially tunable as experimentally demonstrated for dual-color SMLM in cells and allows to access additional spatial information in the axial dimension.