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BP: Fachverband Biologische Physik

BP 25: Cell Mechanics II

BP 25.3: Vortrag

Mittwoch, 18. März 2020, 15:30–15:45, HÜL 386

Dynamics of Circular Dorsal Ruffles as a Function of Cellular State — •Merthe Schwachenwald, Julia Lange, Malte Ohmstede, and Hans-Günther Döbereiner — Institut für Biophysik, Universität Bremen

Circular Dorsal Ruffles (CDRs) are dense moving actin structures that play an important role in cell propagation and the uptake of growth factors. The occurrence and propagation of CDRs are controlled by a multitude of different proteins and interconnected signaling pathways, the most prominent and well-understood being Arp2/3-mediated actin branching. Thus the dynamics of CDRs can be manipulated by interfering with actin regulators or actin itself. Further, the kinetics of CDRs are influenced by membrane tension. To quantify the impact of the different players, we investigate the dynamics of CDRs under narrowly-controlled conditions. Experimentally, we ensure even boundary conditions by using microcontact printed substrates to enforce an even cell shape. The influences of different proteins and growth-factor stimulation on CDR dynamics are controlled employing a microfluidic set-up, and examined using light microscopy. The dynamics of the occurring CDRs are analyzed via kymographs or the use of machine-learning alghorithms.