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BP: Fachverband Biologische Physik
BP 25: Cell Mechanics II
BP 25.7: Vortrag
Mittwoch, 18. März 2020, 16:45–17:00, HÜL 386
Quantification of size-dependent organelle transport in cells — •Simon Wieland1, David Gitschier1, Marius M Kaiser1, Solange Hoffbauer1, Magdalena Haaf1, Adam G Hendricks2, and Holger Kress1 — 1Universität Bayreuth, Arbeitsgruppe Biologische Physik, Bayreuth — 2McGill University, Department of Bioengineering, Montreal
Intracellular organelle transport is a vital process for a large variety of cellular functions, like exocytosis and endocytosis, including phagocytosis. Recently, it was found that organelle transport during phagocytosis is not only regulated biochemically, but also by the size of the organelle [1]: In macrophages, larger phagosomes are transported very persistently towards the nucleus, whereas smaller phagosomes exhibit highly irregular motion. To unravel the molecular causes of this behavior, we systematically quantified the size-dependence of the intracellular transport of phagosomes. Using magnetic tweezers, we found that intracellular transport forces of organelles strongly depend on the cargo size. Immunofluorescence experiments performed on isolated phagosomes allowed us to identify and partially quantify dynein, kinesin-1, and kinesin-2 on the organelles. The scaling behavior of the numbers of dyneins on the organelles together with the scaling behavior of measured stall forces implies cooperation between the molecular motors. These findings can lead to a more fundamental understanding of intracellular transport and the dynamics of molecular motors.
[1]: Keller, S., Berghoff, K., & Kress, H. (2017). Scientific reports, 7(1), 17068.