Dresden 2020 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 26: Focus: Biological Cells in Microfluidics I

BP 26.3: Vortrag

Mittwoch, 18. März 2020, 15:30–15:45, SCH A251

Optical Detection, Characterization and Sorting of Cells and Vesicles in Microfluidics — •Tobias Neckernuss1,2, Daniel Geiger1,2, Jonas Pfeil1,2, Lisa Kwapich1, Patricia Schwilling1, and Othmar Marti11Institute of Experimental Physics, Ulm University — 2Sensific GmbH

High-speed video microscopy is used for many particle or cell detection applications. In order to measure the mechanical properties of cells or to identify their type. With standard equipment none of these tasks can be accomplished in a real-time and in a label-free environment, let alone if a subsequent sorting step is required.
With our new ODIN technology, we are able to assess important parameters like size, shape, velocity, and morphology of different kinds of particles in microfluidic channels and in Lab-on-a-Chip environments. The optical and label-free measurement delivers relevant parameters immediately after the particle passes the sensor. Due to the fixed latency times we are able to set a trigger signal as soon as the particle or cell matches freely configurable, predefined parameters. ODIN enables new measurement applications for particles and cells, leading to potentially groundbreaking changes in Lab-on-a-Chip designs and their throughputs. We present different experiments and sorting tasks regarding cell stiffness, droplet size, particle encapsulation, bacterial analysis, and antibiotic screening performed with ODIN. Simultaneous real-time detection, categorization and sorting enables novel multifunctional Lab-on-a-Chip designs leading to a multifunctional microfluidic factory.

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