Dresden 2020 – wissenschaftliches Programm
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BP 26.8: Vortrag
Mittwoch, 18. März 2020, 17:15–17:30, SCH A251
ROS induces intracellular acidosis associated with increased cell stiffening — •Yesaswini Komaragiri1, Huy Tung Dau1, Doreen Biedenweg2, Ricardo H Pires1, and Oliver Otto1 — 1Biomechanics, ZIK-HIKE, University of Greifswald, Greifswald, Germany — 2University medicine Greifswald, Greifswald, Germany
Reactive oxygen species (ROS) are a primary source of superoxides associated with important alterations in cell physiology. Here, it is accepted that ROS affect the cytoskeleton, however, the interplay with cell mechanics has not been thoroughly investigated. This study focuses on understanding the impact of oxidative stress on the mechanical properties of the human myeloid precursor cell line (HL-60). Generation of ROS was induced by exposing cells to varying concentrations of hydrogen peroxide (H2O2). Using real-time fluorescence deformability cytometry we coupled the mechanical characterization of cells with simultaneous fluorometric assessment of intracellular ROS levels. Our work reveals a direct correlation between the elastic modulus of cells and increased levels of superoxides. Interestingly, the changes in the mechanical phenotype cannot be explained by altered structured of F-actin and microtubule. We demonstrate that cell stiffening at elevated levels of ROS is driven by intracellular acidosis and a corresponding decrease in the cytoplasmic pH of our model system.