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BP: Fachverband Biologische Physik
BP 30: Cell Adhesion and Migration, Multicellular Systemadhesion and Migration, Multicellular Systems II
BP 30.5: Vortrag
Donnerstag, 19. März 2020, 10:45–11:00, HÜL 386
Complex fluid flow, cell polarity and cilia beating patterns in the brain ventricles — •Christian Westendorf1, Shoba Kapoor2, Yong Wang1, Gregor Eichele2, and Eberhard Bodenschatz1 — 1Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077, Goettingen. — 2Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Goettingen.
The brain ventricles are filled with cerebrospinal fluid (CSF) and are lined with a specialized cilia bundle carrying epithelium. The spatially organized beating of the cilia creates CSF flows along the epithelial surface. Particle tracking shows that these flows are very complex, forming a network of flows that varies little between individual mice (Faubel et al., Science, 2016). Using immunohistochemistry with suitable antibodies, we now show that the flow pattern is grounded on the translational and rotational polarity of the epithelial cells. For example whirl like flows are created above cells, whose cilia bundles are oriented accordingly. Additionally, these investigations revealed highly regular patterns in cell shape and cell size, and eccentricity and orientation of the cilia bundles. We further imaged the beating cilia with DIC microscopy with high spatial and temporal resolution over the expanse of the entire ventricular wall. This allowed us to determine the beating properties of cilia and the coordination of beating between the cilia bundles. Alltogether these data suggest that genetic factors make a major contribution to the organization of the flow patterns along the ventricular wall.