Dresden 2020 – wissenschaftliches Programm
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BP 5.1: Hauptvortrag
Montag, 16. März 2020, 15:00–15:30, HÜL 386
Mirror-enhanced fluorescence for superresolution imaging and spectroscopy — •Katrin G. Heinze1, Hannah S. Heil1, Benjamin Schreiber1, and Markus Sauer2 — 1Rudolf Virchow Center, University of Würzburg, Würzburg, Germany — 2Biocenter of the University of Würzburg, Würzburg, Germany
The "Resolution Revolution" in fluorescence microscopy over the last decade has given rise to a variety of techniques that allow imaging with resolution up to the nanometer range. One remarkable technique is direct stochastic optical reconstruction microscopy (dSTORM), a widely-used type of single molecule localization microscopy (SMLM). The key point here is the achievable localization precision, which mainly depends on the image contrast generated by the individual fluorophore*s emission. We found that reflective metal-dielectric nano-coatings represent a tunable nano-mirror that can do both quenching and boosting fluorescence for high-contrast imaging on the nanoscale. The enhanced resolution is a near-field effect and thus restricted to surface imaging; however, most membrane fluorescence applications benefit, even if classic resolution is not the main concern: Spectroscopic methods in live-cells such as Fluorescence Correlation Spectroscopy and Fluorescence Resonance Energy Transfer also belong to the scope of application. Mirror-enhanced fluorescence is different from other surface methods based on total internal reflection microscopy or optoplasmonics. While surface-plasmon supported methods provide much higher enhancement factors, mirror-enhanced approaches are more versatile and thus highly suitable for modern bio-imaging.