Dresden 2020 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 9: Poster II

BP 9.10: Poster

Montag, 16. März 2020, 17:30–19:30, P2/2OG

Investigation of transport behavior of multiple kinesin-3 motors coupled directly to membranous cargoAshwin D'souza1, •Rahul Grover1, and Stefan Diez1,21B CUBE, Center for Molecular Bioengineering, TU Dresden, Germany — 2Cluster of Excellence Physics of Life, TU Dresden, Germany

KIF16B, a kinesin-3 family motor protein, can bind directly to phosphatidylinositols (PI3P) containing organelles such as early endosome and transport them, on microtubules, towards cell periphery. Recently, it was shown that the motor-membrane coupling can influence the transport efficiency of the cargo, dependent on the motor density and membrane fluidity. However, these studies were performed on a planar solid supported lipid bilayer, thus the influence of the cargo shape and size on the transport behavior were not determined. Here, we explore the behavior of ensembles of KIF16B motors transporting spherical liposomes of varying membrane fluidity and size, with a range of surface motor densities. We found that liposomes transported by multiple KIF16Bs have lower velocities compared to the stepping velocity of single KIF16B motors. Moreover, liposomes exhibited higher velocities at lower surface densities of KIF16B, compared to when being carried by higher motor densities. Liposomes driven by ensembles of KIF16B motors also exhibited stop-and-go motion, i.e. processive runs interrupted by pauses which could be an outcome of asynchronous stepping of individual KIF16B motors within an ensemble. This behavior appears to be an emergent property of multi-motor transport as the frequency of pauses for single KIF16B motors is much lower than the liposomes.

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