BPCPPDYSOE21 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 24: Poster B: Active Biological Matter, Cell Mechanics, Systems Biology, Computational Biophysics, etc.
BP 24.14: Poster
Dienstag, 23. März 2021, 16:00–18:30, BPp
Growing with vacancies: Eden growth models suggest that flat clathrin lattices assemble with spatial heterogeneity — •Felix Frey1,2, Delia Bucher3, Kem A. Sochacki4, Justin W. Taraska4, Steeve Boulant3, and Ulrich S. Schwarz1 — 1ITP and BioQuant, Heidelberg University, DE — 2Department of Bionanoscience, TU Delft, NL — 3CIID, University Hospital Heidelberg and DKFZ, DE — 4NHLBI, NIH, Bethesda, US
Biological cells constantly transport material across their plasma membrane and clathrin-mediated endocytosis is one of the main uptake mechanisms. Recently, it has been shown that clathrin lattices first assemble flat before the clathrin-coated membrane starts to invaginate [1]. However, how this flat-to-curved transition proceeds in detail is still unclear, since energetic and topological barriers exist and it is difficult to observe the assembly process in time and space. Here we hypothesize that clathrin lattices grow with lattice vacancies that would facilitate the flat-to-curved transition. We identify the Eden growth model as the most suitable framework for clathrin lattice growth. We then derive four distinct variants of the model that represent the different binding modes of clathrin triskelia based on their geometry. Our computer simulations show that the different model variants lead to distinct lattice shapes and densities. Comparison with experimental electron microscopy and correlative light microscopy data suggests that clathrin lattices grow with a moderate level of lattice vacancies [2]. [1] D. Bucher*, F. Frey*, et al., Nat. Communs. 9, 1109 (2018). [2] F. Frey et al., New J. of Phys. 22, 073043 (2020).