Erlangen 2022 – wissenschaftliches Programm

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MS: Fachverband Massenspektrometrie

MS 2: Mass Spectrometry Methods

MS 2.2: Vortrag

Montag, 14. März 2022, 17:00–17:15, MS-H9

A digital RF ion filter and trap combination for the MS SPIDOC prototype — •Florian Simke, Paul Fischer, and Lutz Schweikhard — Universität Greifswald, Institut für Physik, Felix-Hausdorff-Str. 6, 17489 Greifswald

The MS SPIDOC (Mass Spectrometry for Single Particle Imaging of Dipole Oriented protein Complexes) prototype [1] will deliver mass- and conformation separated samples of protein-based biomolecules for single-particle imaging analysis at the European X-Ray Free-Electron Laser Facility (XFEL)[2]. The design, simulation, and construction of one of its key modules is presented along with first offline results. The module consists of a linear-quadrupole filter assembly and a linear-quadrupole ion trap, both operated with digital radio frequencies. The restriction to keep investigated biomolecules as native as possible leads to a design that separates ion filtering and accumulation/trapping. The module is able to filter ions of interest with mass-to-charge ratios of up to m/z≈12000 Th. The ion trap is utilized to collect and bunch incoming ions from a continuous source and eject them with a narrow temporal width for downstream analysis and X-ray interaction.

[1] C. Uetrecht et al., Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging, Journal of Synchrotron Radiation 26 (3) (2019) 653-659. doi:10.1107/S1600577519002686
[2] Europe turns on bright x-ray source, Nature Photon; 11 (2017) 609-609, doi:https://doi.org/10.1038/s41566-017-0025-z